Scientific Programme


The scientific programme will start on Monday morning, 24th September, and finish on Wednesday afternoon, 26th September, 2018. The symposium will have 10 sessions on which will be presented 11 Invited Lectures (INV, 30 min) and 34 Topical Lectures (15 min) complemented by 1 Poster Session (posters will be on all 3-days). In Monday evening the Consortium meeting will take place. See the schedule of scientific program below and session block schedule.





SUNDAY    |    MONDAY    |    TUESDAY    |    WEDNESDAY





SUNDAY, 23RD SEPTEMBER 2018


15:00 - 19:00

Registration

18:00 - 21:00

WELCOME RECEPTION (Hotel DUO, Meeting Hall Berlin)





MONDAY, 24TH SEPTEMBER 2018


08:00 - 11:00

Registration

08:45 - 09:00

WELCOME AND OPENING OF BIOELECTRICS 2018
Petr LUKES, Chair of BIOELECTRICS 2018
Radomir PANEK, director IPP CAS


09:00 - 10:00

SESSION 1: BIOLOGICAL RESPONSES (MOLECULAR, SUBCELLULAR, CELLULAR AND INTERCELLULAR) I
Session Chair: Gregor SERSA, Institute of Oncology Ljubljana, Ljubljana, Slovenia

09:00 - 09:30

INV 1: Gene Electrotransfer as an Effective Approach to Deliver Plasmids Encoding Immune Modulators for Treatment of Melanoma
Richard HELLER, Frank Reidy Research Center For Bioelectrics, Old Dominion University, USA

09:30 - 10:00

INV 2: Calcium Electroporation: Preclinical and Clinical Results, with a Look Towards Future Perspectives
Julie GEHL, University of Copenhagen, Denmark


10:00 - 10:30

COFFEE BREAK


10:30 - 12:00

SESSION 2: BIOLOGICAL RESPONSES (MOLECULAR, SUBCELLULAR, CELLULAR AND INTERCELLULAR) II
Session Chair: Andrei G. PAKHOMOV, Frank Reidy Research Center For Bioelectrics, Old Dominion University, USA

10:30 - 11:00

INV 3: Monitoring the Membrane Permeabilization and Molecular Composition of Live Cells Exposed to Pulsed Electric Fields based on Terahertz Measurements
Lluis M. MIR, CNRS VAT, Villejuif, France

11:00 - 11:15

ZBP1/DAI, a DNA-Specific Pattern Recognition Receptor, Binds Plasmid DNA Directly After Electroporation
Loree HELLER, Frank Reidy Research Center For Bioelectrics, Old Dominion University, USA

11:15 - 11:30

How Imaging Membrane and Cell Processes Involved in Electropermeabilization Can Improve Its Development in Cell Biology and in Clinics
Marie-Pierre ROLS, IPBS-CNRS, Toulouse, France

11:30 - 11:45

Activation of Voltage-Gated Calcium Channels by Uni- and Bipolar 300-ns Pulses
Olga PAKHOMOVA, Frank Reidy Research Center For Bioelectrics, Old Dominion University, USA

11:45 - 12:00

Optical Recording of Action Potentials in E18 Hippocampal Neurons Exposed to Ultrashort Pulsed Electric Fields
Iurii SEMENOV, Frank Reidy Research Center For Bioelectrics, Old Dominion University, USA


12:00 - 13:30

LUNCH


13:30 - 15:30

SESSION 3: BIOPHYSICS AND BIOCHEMISTRY OF INTERACTION MECHANISMS
Session Chair: Sunao KATSUKI, Kumamoto University, Japan

13:30 - 14:00

INV 4: Nanoscale Transport Through Permeabilizing Structures in Electrically Stressed Cell Membranes
P. Thomas VERNIER, Frank Reidy Research Center For Bioelectrics, Old Dominion University, USA

14:00 - 14:30

INV 5: Bipolar Cancellation Under "Classical" Electropermeabilization
Justin TEISSIE, IPBS-CNRS, Toulouse, France

14:30 - 14:45

Intense Electric Field Affects C-Terminus of Tubulin: Molecular Dynamics Simulation Study
Michal CIFRA, Institute of Photonics and Electronics of the Czech Academy of Sciences, Prague, Czech Republic

14:45 - 15:00

Microsecond Kinetics of Ion Transport and Membrane Interface Binding Before, During, and After Lipid Electropore Formation
Federica CASTELLANI, Frank Reidy Research Center For Bioelectrics, Old Dominion University, USA

15:00 - 15:15

Endothelial Cell-To-Cell Junctions Alteration and Repair Are Responsible for Transient Permeability of Blood Vessels After Reversible Electroporation
Muriel GOLZIO, IPBS CNRS Toulouse, France

15:15 - 15:30

CellDesigner as a Tool for the Analysis of the Kinetics of Pore Formation and Disappearance during Cell Electroporation
Gintautas SAULIS, Vytautas Magnus University, Kaunas, Lithuania


15:30 - 16:00

COFFEE BREAK


16:00 - 17:00

POSTER SESSION  (List of Posters)
Session Chair: Stine Krog FRANDSEN, Center for Experimental Drug and Gene Electrotransfer, Zealand University Hospital, Denmark


17:00 - 18:00

CONSORTIUM MEETING (Hotel DUO, Conference Hall Prague)


19:30 - 22:00

CONSORTIUM DINNER (Prague Downtown)




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TUESDAY, 25TH SEPTEMBER 2018


09:00 - 10:00

SESSION 4: BIOLOGICAL RESPONSES, CANCER TREATMENT AND TUMOR ABLATION
Session Chair: Richard HELLER, Frank Reidy Research Center For Bioelectrics, Old Dominion University, USA

09:00 - 09:30

INV 6: Second Phase Amplitude Determines the Efficiency of Electroporation by Bipolar Nanosecond Pulses
Andrei G. PAKHOMOV, Frank Reidy Research Center For Bioelectrics, Old Dominion University, USA

09:30 - 10:00

INV 7: Some Predictive Factors for the Response of Tumors to Electrochemotherapy: Preclinical Studies
Gregor SERSA, Institute of Oncology Ljubljana, Ljubljana, Slovenia


10:00 - 10:30

COFFEE BREAK


10:30 - 12:15

SESSION 5: CANCER TREATMENT AND TUMOR ABLATION
Session Chair: Julie GEHL, University of Copenhagen, Denmark

10:30 - 11:00

INV 8: Detecting the Changes in Gene Eexpression in Murine Melanoma Tumors Treated With Nano-Pulse Stimulation (NPS) Using NanoString Technology
Richard NUCCITELLI, Pulse Biosciences, USA

11:00 - 11:15

Electrochemotherapy of Porcine Liver: Assessment of Systemic and Local Side Effects
Maja CEZAMAR, Institute of Oncology Ljubljana, Ljubljana, Slovenia

11:15 - 11:30

Lipid Composition of the Plasma Membrane and Calcium Electroporation Efficacy
Stine Krog FRANDSEN, Center for Experimental Drug and Gene Electrotransfer, Zealand University Hospital, Denmark

11:30 - 11:45

Mechanisms of NanoPulse Stimulation-Induced Immunity in Hepatocellular Carcinoma and Mammary Cancer
Stephen J. BEEBE, Frank Reidy Research Center For Bioelectrics, Old Dominion University, USA

11:45 - 12:00

Mechanisms and Immunogenicity of nsPEF-Induced Cell Death in B16F10 Melanoma Tumors
Claudia MURATORI, Frank Reidy Research Center For Bioelectrics, Old Dominion University, USA

12:00 - 12:15

Combination of Pulsed Electric Fields and Cold Atmospheric Plasma for Cancer Treatment
Christina WOLFF, Leibniz Institute for Plasma Science and Technology, Greifswald, Germany


12:15 - 13:45

LUNCH


13:45 - 15:30

SESSION 6: BIOLOGICAL RESPONSES (MOLECULAR, SUBCELLULAR, CELLULAR AND INTERCELLULAR) III
Session Chair: Lluis M. MIR, CNRS VAT, Villejuif, France

13:45 - 14:00

In Situ Tumor Vaccination by TNFα Combined With Immune Boost by IL-12 Using Concomitant Intratumoral Gene Electrotransfer
Masa Bosnjak, Institute of Oncology Ljubljana, Slovenia

14:00 - 14:15

Structural Change of Multimer Proteins Exposed to 300 kV/cm Electrical Pulses
Gen Urabe, Kumamoto University, Japan

14:15 - 14:30

"Cancellation of Cancellation" (CANCAN) - Remote Electroporation by Nanosecond Bipolar Electric Pulses
Elena GIANULIS, Frank Reidy Research Center For Bioelectrics, Old Dominion University, USA

14:30 - 14:45

Electroporative Membrane Depolarization as a Mechanism for nsPEF-Induced Voltage-Gated Calcium Channel Opening
Kiril HRISTOV, Frank Reidy Research Center For Bioelectrics, Old Dominion University, USA

14:45 - 15:00

Observations of Transport and Cancellation of Transport into Electrically Stressed Cells Defy "The Standard Model"
Esin SOZER, Frank Reidy Research Center For Bioelectrics, Old Dominion University, USA

15:00 - 15:15

Long-Term Development of Cardiac Lesions Created with Nanosecond Pulsed Electric Fields in a Preclinical Pig Survival Model
Christian ZEMLIN, Frank Reidy Research Center For Bioelectrics, Old Dominion University, USA

15:15 - 15:30

Mitochondrial Ca2+ Uptake after the Exposure to a Single Microsecond Pulse
Nobuaki OHNISHI, Kumamoto University, Kumamoto, Japan


15:30 - 16:00

COFFEE BREAK


16:00 - 17:00

SESSION 7: BIOLOGICAL RESPONSES (MOLECULAR, SUBCELLULAR, CELLULAR AND INTERCELLULAR) IV
Session Chair: P. Thomas VERNIER, Frank Reidy Research Center For Bioelectrics, Old Dominion University, USA

16:00 - 16:15

Improvement in Electrotransfection of CHO Cells by Varying the Plasmid Concentration
Sonam CHOPRA, Vytautas Magnus University, Kaunas, Lithuania

16:15 - 16:30

Monopolar Electrotransfer Enhances Gene Delivery in Vivo
Anna BULYSHEVA, Frank Reidy Research Center For Bioelectrics, Old Dominion University, USA

16:30 - 16:45

The Effect of Calcium Ions Delivered by Electroporation on ATP Consumption and Cell Viability of Breast Adenocarcinoma Cell Lines
Jolanta SACZKO, Wroclaw Medical University, Poland

16:45 - 17:00

Nanoelectropulse Delivery for Cell Membrane Perturbation and Oxidation
Julita KULBACKA, Wroclaw Medical University, Poland


18:30 - 19:00

CONFERENCE BANQUET RECEPTION (Hotel DUO)

19:00 - 23:00

CONFERENCE BANQUET (Hotel DUO, Meeting Hall Amsterdam)



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WEDNESDAY, 26TH SEPTEMBER 2018


09:00 - 10:00

SESSION 8: BIOMASS TREATMENT
Session Chair: Justin TEISSIE, IPBS-CNRS, Toulouse, France

09:00 - 09:30

INV 10: Progress on Bioelectrics Activities in Microalgae Processing and in Bacterial Decontamination at KIT
Wolfgang FREY, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany

09:30 - 10:00

INV 11: Non-thermal Plasmas for Pre- and Post-harvest Processing of Seeds and Crops
Juergen F. KOLB, Leibniz Institute for Plasma Science and Technology, Greifswald, Germany


10:00 - 10:30

COFFEE BREAK


10:30 - 12:15

SESSION 9: EXPERIMENTAL TECHNIQUES, DIAGNOSTICS AND METHODS
Session Chair: Juergen F. KOLB, Leibniz Institute for Plasma Science and Technology, Greifswald, Germany

10:30 - 11:00

INV 12: Study of Adherent Cell Permeabilization in Various Plasma Gun Plasma Delivery Protocols
Eric ROBERT, GREMI, Orleans, France

11:00 - 11:15

Flexible (Sub-)Nanosecond High-Voltage Pulse Generation
Guus PEMEN, Eindhoven University of Technology, The Netherlands

11:15 - 11:30

Label-Free Endogenous Chemiluminescence for Probing Oxidative Effects of Electric Pulses
Michal CIFRA, Institute of Photonics and Electronics of the Czech Academy of Sciences, Prague, Czech Republic

11:30 - 11:45

Electropermeabilization and Electrostimulation by Picosecond Pulses
Shu XIAO, Frank Reidy Research Center For Bioelectrics, Old Dominion University, USA

11:45 - 12:00

Impulsive Acceleration for Needle-Free Drug/Vaccine Delivery
Hamid HOSANO, Kumamoto University, Kumamoto, Japan

12:00 - 12:15

High-Voltage Nanosecond Bipolar Pulse Generator with Delayed Positive or Negative Polarity
Philippe LEVEQUE, XLIM - University of Limoges - CNRS, France


12:15 - 13:45

LUNCH


13:45 - 15:00

SESSION 10: ENVIRONMENTAL APPLICATIONS, BIOMASS PROCESSING AND FOOD TREATMENT
Session Chair: Wolfgang FREY, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany

13:45 - 14:00

Impact of Membrane Recovery of Gram-positive and Gram-negative Bacteria on the Inactivation Efficiency of Pulsed Electric Field Treatment
Christian GUSBETH, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany

14:00 - 14:15

Pulsed Electric Field (PEF) Assisted Protein Recovery from Microalgae Biomass for Food and Feed Applications
Daniel SHERER, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany

14:15 - 14:30

Cell Wall Rupture of Microalgae for the Extraction of Metabolites by Applying Spark Discharges Effects and Mechanism
Katja ZOCHER, Leibniz Institute for Plasma Science and Technology, Greifswald, Germany

14:30 - 14:45

Effect of Frequency in Extraction of the Yeast-Derived Lactase Using Burst Pulse High Electric Field
Yasushi MINAMITANI, Yamagata University, Yonezawa, Japan

14:45 - 15:00

Enhancement of Vitality and Activity of a Plant Growth-Promoting Bacteria (PGPB) by Atmospheric Pressure Non-Thermal Plasma
Sang-Hye JI, National Fusion Research Institute, Gunsan, Republic of Korea


15:00 - 15:30

CONFERENCE CLOSING & COFFEE




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